NAD+
Updated April 28, 2026
Why People Choose NAD+
• Supports cellular energy production.
• Helps support mitochondrial function.
• Promotes vitality and recovery.
• May support healthy aging when used as part of a structured protocol.
NAD+ Mechanism of Action
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme found in every cell and is central to energy metabolism. It helps shuttle electrons during mitochondrial ATP production and also serves as a required substrate for enzymes involved in cellular repair, stress response, and metabolic regulation. By supporting these core processes, NAD+ may help promote cellular energy output, recovery, and healthy aging.
Reconstitution (Mixing)
• NAD+ 500 mg vial - add 4 mL bacteriostatic water.
• Gently swirl or roll the vial to mix. Do not shake.
• With this mixture, 40 units in a 1 mL insulin syringe provides 50 mg.
Dosing
• Method: Subcutaneous (SQ) injection
• Exact Units Per Injection: 40 units (50 mg)
• Frequency: Once every 3rd day
• Cycle Length: 4 weeks
• Timing: Take in the morning on an empty stomach, at least 30 minutes before eating.
Injection
• Use a 1 mL insulin syringe (30-31G, 6-8 mm) for subcutaneous injections.
• Preferred SQ sites include the lower abdomen, outer thigh, or upper buttock area.
• Rotate injection sites to reduce irritation.
1. Wash your hands and clean the vial top and injection site with an alcohol swab.
2. Draw the prescribed dose into the syringe.
3. Pinch the skin, insert the needle into the fatty tissue, and inject slowly.
4. Remove the needle, apply light pressure if needed, and safely discard the syringe in a sharps container.
Peptide Intake Overview
• Per injection: 50 mg
• Schedule: once every 3rd day for 4 weeks
• Approximate total use over 4 weeks: 10 injections = 500 mg total
Notes
• Morning use is preferrable.
• Using NAD+ on an empty stomach can improve uptake and effectiveness.
• Stay consistent with the every-3rd-day schedule across the full 4-week cycle.
Possible Side Effects (Common & Mild)
• Mild redness, irritation, or tenderness at the injection site.
• Temporary nausea or stomach discomfort in some individuals.
• Mild headache or flushing in sensitive individuals.
• If side effects feel persistent or significant, pause use and speak with a licensed healthcare professional.
Disclaimer
The information provided in this article is for educational and informational purposes only and is not intended as medical advice. Peptides and other compounds discussed are intended for research purposes only and are not approved for human consumption unless prescribed by a licensed medical professional. Always consult your healthcare provider before starting any new protocol, supplement, or treatment.